Journal: Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc

Article Title: Improved detection of Mycobacterium tuberculosis and M. bovis in African wildlife samples using cationic peptide decontamination and mycobacterial culture supplementation

doi: 10.1177/10406387211044192

Figure Lengend Snippet: Conventional Bactec MGIT (MGIT tube containing PANTA-OADC antimicrobial enrichment media) and TiKa (MGIT tube containing PANTA-OADC and TiKa supplement B) culture results represented as median time to positivity (d) with interquartile ranges (IQR) for sterile phosphate buffer spiked in duplicate with known amounts (cfu) of A. Mycobacterium bovis SB0121 (10,000–10 cfu) and B. M. tuberculosis H37Rv (10,000–10 cfu), respectively. Prior to inoculation into growth indicator tubes, all spiked samples were either: decontaminated with MycoPrep (treatment 1: MycoPrep MGIT; treatment 2: MycoPrep TiKa), not decontaminated (treatment 3: MGIT control; treatment 4: TiKa control), or decontaminated with KiC agent (treatment 5: KiC MGIT; treatment 6: KiC TiKa). For some data points, the IQRs were shorter than the height of the bar; in these cases, the ranges were not drawn. Using the multiple t -test with Bonferroni–Dunn correction for multiple testing (alpha = 0.01), statistical significance ( p < 0.01) was calculated between treatments for each amount of mycobacteria. Within each amount of the target Mycobacterium , bars with a different superscript are significantly different at p < 0.01.

Article Snippet: TiKa liquid cultures used 7-mL MGIT tubes containing 800 μL of antimicrobial growth supplement mixture (PANTA + OADC; Becton Dickinson), 8.5 μL of growth supplement B (TiKa Diagnostics), and 500 μL of each treated sample.

Techniques: Sterility, Control

Journal: Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc

Article Title: Improved detection of Mycobacterium tuberculosis and M. bovis in African wildlife samples using cationic peptide decontamination and mycobacterial culture supplementation

doi: 10.1177/10406387211044192

Figure Lengend Snippet: Comparative mycobacterial culture results for tissue and respiratory samples (bronchial alveolar lavage fluid and trunk washes) collected from 26 known Mycobacterium tuberculosis complex (MTBC)-infected African buffaloes, white rhinoceros (25 samples from 6 animals), and African elephants (3 samples from 2 animals) from bovine tuberculosis endemic wildlife parks and a zoo (1 elephant) within South Africa.

Article Snippet: TiKa liquid cultures used 7-mL MGIT tubes containing 800 μL of antimicrobial growth supplement mixture (PANTA + OADC; Becton Dickinson), 8.5 μL of growth supplement B (TiKa Diagnostics), and 500 μL of each treated sample.

Techniques: